In and ex vivo imaging
Main Fields of Research
The main focus of the in vivo imaging group is to establish, develop and use modern non-invasive imaging techniques like optical coherence tomography, confocal laser microscopy and line-field confocal OCT to optimize the detection and early diagnostics of melanoma and non-melanoma skin cancer, but also for inflammatory and infectious dermatoses. In new prototype devices, we recognize and establish new patterns for diagnosis to improve sensitivity and specificity of the clinical diagnosis and correlate these findings to ex vivo imaging and histopathology results.
These methods are non-invasive without pain, surgery, local anaesthesia, scarring etc. but yet allow a precise diagnosis in real-time and therefore can spare unnecessary biopsies or excisions or speed up referral to the right treatment. As the same skin site can be visualized over time e.g. before and after treatment, these methods are also valuable tools for therapy monitoring.
Ex vivo confocal laser scanning microscopy (ex vivo CLSM) is a novel diagnostic method allowing rapid, high-resolution imaging of freshly excised skin samples in an intraoperative setting. It enables the surgeon to know the diagnosis and whether the resection edges are clear or not within few minutes after the excision. This innovative bedside histology may decrease the number of surgical procedures and length of hospitalization in the near future.
Prof. Elke Sattler, MD
Prof. Daniela Hartmann, MD, Ph.D.
Cristel Ruini, MD – deputy lead
Ann-Charlotte Niesert, MD
Isin Sinem Bağcı, MD
Michaela Kubiniec, MD
Leonie Frommherz, MD
Sebastian Krammer, MD
Stephanie Steckmeier, MD
Martina Zacher, MD
Charlotte Gust, MD student
Fabia Daxenberger, MD student
Natalie Schädle, MD student
Gabriela Vladimirova, MD student
Sophia Schlingmann, MD student
Elena Kunrad, MD student
Kathrin Patzer, MD student
Marie Düsedau, MD student
Ramona Fenderle; MD student
Ex vivo imaging
» Bağcı IS, Aoki R, Vladimirova G, Ergün E, Ruzicka T, Sárdy M, French LE, Hartmann D. New generation diagnostics in inflammatory skin diseases: immunofluorescence and histopathological assessment using ex vivo confocal laser scanning microscopy in cutaneous lupus erythematosus. Exp Dermatol 2020 Dec 21
» Hartmann D, Krammer S, Vural S, Bachmann MR, Ruini C, Sárdy M, Ruzicka T, Berking C, von Braunmühl T. Immunofluorescence and confocal microscopy for exvivo diagnosis of melanocytic and non-melanocytic skin tumors: A pilot study. J Biophotonics 2018 Mar;11(3)
In vivo imaging
» Ruini C, Schuh S, Sattler E*, Welzel J*. Line-field confocal optical coherence tomography-Practical applications in dermatology and comparison with established imaging methods. Skin Res Technol 2020 Oct 21
» Sattler EC, Hoffmann VS, Ruzicka T, Maier T, Berking C. Reflectance confocal microscopy for monitoring the density of demodex mites in rosacea patients before and after treatment. Br J Dermatol 2015 ; 173(1):69-75